PhytophthoraV1, Main, Exploration, bibRecord, 000253

Design of Fungal Co-Cultivation Based on Comparative Metabolomics and Bioactivity for Discovery of Marine Fungal Agrochemicals.

Identifieur interne : 000253 ( Main/Exploration ); précédent : 000252; suivant : 000254

Design of Fungal Co-Cultivation Based on Comparative Metabolomics and Bioactivity for Discovery of Marine Fungal Agrochemicals.

Auteurs : Ernest Oppong-Danquah [Allemagne] ; Paulina Budnicka [Allemagne] ; Martina Blümel [Allemagne] ; Deniz Tasdemir [Allemagne]

Source :

Marine drugs [ 1660-3397 ] ; 2020.

RBID : pubmed:31979232

Descripteurs français

KwdFr :
- Agrochimie (composition chimique), Agrochimie (isolement et purification), Agrochimie (métabolisme), Agrochimie (pharmacologie), Champignons (isolement et purification), Champignons (métabolisme), Concentration inhibitrice 50 (MeSH), Microbiologie industrielle (méthodes), Métabolomique (MeSH), Organismes aquatiques (isolement et purification), Organismes aquatiques (métabolisme), Phytophthora infestans (effets des médicaments et des substances chimiques), Plan de recherche (MeSH), Polycétides (isolement et purification), Polycétides (métabolisme), Produits biologiques (composition chimique), Produits biologiques (isolement et purification), Produits biologiques (métabolisme), Produits biologiques (pharmacologie), Spectroscopie par résonance magnétique (MeSH), Sédiments géologiques (microbiologie), Techniques de coculture (méthodes), Xanthomonas campestris (effets des médicaments et des substances chimiques).
MESH :
- composition chimique : Agrochimie, Produits biologiques.
- effets des médicaments et des substances chimiques : Phytophthora infestans, Xanthomonas campestris.
- isolement et purification : Agrochimie, Champignons, Organismes aquatiques, Polycétides, Produits biologiques.
- microbiologie : Sédiments géologiques.
- métabolisme : Agrochimie, Champignons, Organismes aquatiques, Polycétides, Produits biologiques.
- méthodes : Microbiologie industrielle, Techniques de coculture.
- pharmacologie : Agrochimie, Produits biologiques.
- Concentration inhibitrice 50, Métabolomique, Plan de recherche, Spectroscopie par résonance magnétique.

English descriptors

KwdEn :
- Agrochemicals (chemistry), Agrochemicals (isolation & purification), Agrochemicals (metabolism), Agrochemicals (pharmacology), Aquatic Organisms (isolation & purification), Aquatic Organisms (metabolism), Biological Products (chemistry), Biological Products (isolation & purification), Biological Products (metabolism), Biological Products (pharmacology), Coculture Techniques (methods), Fungi (isolation & purification), Fungi (metabolism), Geologic Sediments (microbiology), Industrial Microbiology (methods), Inhibitory Concentration 50 (MeSH), Magnetic Resonance Spectroscopy (MeSH), Metabolomics (MeSH), Phytophthora infestans (drug effects), Polyketides (isolation & purification), Polyketides (metabolism), Research Design (MeSH), Xanthomonas campestris (drug effects).
MESH :
- chemical , chemistry : Agrochemicals, Biological Products.
- chemical , isolation & purification : Agrochemicals, Biological Products, Polyketides.
- chemical , metabolism : Agrochemicals, Biological Products, Polyketides.
- chemical , pharmacology : Agrochemicals, Biological Products.
- drug effects : Phytophthora infestans, Xanthomonas campestris.
- isolation & purification : Aquatic Organisms, Fungi.
- metabolism : Aquatic Organisms, Fungi.
- methods : Coculture Techniques, Industrial Microbiology.
- microbiology : Geologic Sediments.
- Inhibitory Concentration 50, Magnetic Resonance Spectroscopy, Metabolomics, Research Design.

Abstract

Microbial co-cultivation is employed for awakening silent biosynthetic gene clusters (BGCs) to enhance chemical diversity. However, the selection of appropriate partners for co-cultivation remains a challenge. Furthermore, competitive interactions involving the suppression of BGCs or upregulation of known, functional metabolite(s) during co-cultivation efforts is also common. Herein, we performed an alternative approach for targeted selection of the best co-cultivation pair. Eight marine sediment-derived fungi were classified as strong or weak, based on their anti-phytopathogenic potency. The fungi were co-cultured systematically and analyzed for their chemical profiles and anti-phytopathogenic activity. Based on enhanced bioactivity and a significantly different metabolite profile including the appearance of a co-culture specific cluster, the co-culture of Plenodomusinfluorescens (strong) and Pyrenochaetanobilis (weak) was prioritized for chemical investigation. Large-scale co-cultivation resulted in isolation of five polyketide type compounds: two 12-membered macrolides, dendrodolide E (1) and its new analog dendrodolide N (2), as well as two rare azaphilones spiciferinone (3) and its new analog 8a-hydroxy-spiciferinone (4). A well-known bis-naphtho-γ-pyrone type mycotoxin, cephalochromin (5), whose production was specifically enhanced in the co-culture, was also isolated. Chemical structures of compounds 1-5 were elucidated by NMR, HRMS and [] analyses. Compound 5 showed the strongest anti-phytopathogenic activity against Xanthomonas campestris and Phytophthorainfestans with IC₅₀ values of 0.9 and 1.7 µg/mL, respectively.

DOI: 10.3390/md18020073
PubMed: 31979232
PubMed Central: PMC7073616

Affiliations:

Links toward previous steps (curation, corpus...)

to stream Main, to step Corpus: 000291
to stream Main, to step Curation: 000291

Le document en format XML

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<term>Agrochemicals (metabolism)</term>
<term>Agrochemicals (pharmacology)</term>
<term>Aquatic Organisms (isolation & purification)</term>
<term>Aquatic Organisms (metabolism)</term>
<term>Biological Products (chemistry)</term>
<term>Biological Products (isolation & purification)</term>
<term>Biological Products (metabolism)</term>
<term>Biological Products (pharmacology)</term>
<term>Coculture Techniques (methods)</term>
<term>Fungi (isolation & purification)</term>
<term>Fungi (metabolism)</term>
<term>Geologic Sediments (microbiology)</term>
<term>Industrial Microbiology (methods)</term>
<term>Inhibitory Concentration 50 (MeSH)</term>
<term>Magnetic Resonance Spectroscopy (MeSH)</term>
<term>Metabolomics (MeSH)</term>
<term>Phytophthora infestans (drug effects)</term>
<term>Polyketides (isolation & purification)</term>
<term>Polyketides (metabolism)</term>
<term>Research Design (MeSH)</term>
<term>Xanthomonas campestris (drug effects)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Agrochimie (composition chimique)</term>
<term>Agrochimie (isolement et purification)</term>
<term>Agrochimie (métabolisme)</term>
<term>Agrochimie (pharmacologie)</term>
<term>Champignons (isolement et purification)</term>
<term>Champignons (métabolisme)</term>
<term>Concentration inhibitrice 50 (MeSH)</term>
<term>Microbiologie industrielle (méthodes)</term>
<term>Métabolomique (MeSH)</term>
<term>Organismes aquatiques (isolement et purification)</term>
<term>Organismes aquatiques (métabolisme)</term>
<term>Phytophthora infestans (effets des médicaments et des substances chimiques)</term>
<term>Plan de recherche (MeSH)</term>
<term>Polycétides (isolement et purification)</term>
<term>Polycétides (métabolisme)</term>
<term>Produits biologiques (composition chimique)</term>
<term>Produits biologiques (isolement et purification)</term>
<term>Produits biologiques (métabolisme)</term>
<term>Produits biologiques (pharmacologie)</term>
<term>Spectroscopie par résonance magnétique (MeSH)</term>
<term>Sédiments géologiques (microbiologie)</term>
<term>Techniques de coculture (méthodes)</term>
<term>Xanthomonas campestris (effets des médicaments et des substances chimiques)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Agrochemicals</term>
<term>Biological Products</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>Agrochemicals</term>
<term>Biological Products</term>
<term>Polyketides</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Agrochemicals</term>
<term>Biological Products</term>
<term>Polyketides</term>
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<keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Agrochemicals</term>
<term>Biological Products</term>
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<keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Agrochimie</term>
<term>Produits biologiques</term>
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<keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Phytophthora infestans</term>
<term>Xanthomonas campestris</term>
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<keywords scheme="MESH" qualifier="effets des médicaments et des substances chimiques" xml:lang="fr"><term>Phytophthora infestans</term>
<term>Xanthomonas campestris</term>
</keywords>
<keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Aquatic Organisms</term>
<term>Fungi</term>
</keywords>
<keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Agrochimie</term>
<term>Champignons</term>
<term>Organismes aquatiques</term>
<term>Polycétides</term>
<term>Produits biologiques</term>
</keywords>
<keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Aquatic Organisms</term>
<term>Fungi</term>
</keywords>
<keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Coculture Techniques</term>
<term>Industrial Microbiology</term>
</keywords>
<keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Sédiments géologiques</term>
</keywords>
<keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Geologic Sediments</term>
</keywords>
<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Agrochimie</term>
<term>Champignons</term>
<term>Organismes aquatiques</term>
<term>Polycétides</term>
<term>Produits biologiques</term>
</keywords>
<keywords scheme="MESH" qualifier="méthodes" xml:lang="fr"><term>Microbiologie industrielle</term>
<term>Techniques de coculture</term>
</keywords>
<keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Agrochimie</term>
<term>Produits biologiques</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Inhibitory Concentration 50</term>
<term>Magnetic Resonance Spectroscopy</term>
<term>Metabolomics</term>
<term>Research Design</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Concentration inhibitrice 50</term>
<term>Métabolomique</term>
<term>Plan de recherche</term>
<term>Spectroscopie par résonance magnétique</term>
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<front><div type="abstract" xml:lang="en">Microbial co-cultivation is employed for awakening silent biosynthetic gene clusters (BGCs) to enhance chemical diversity. However, the selection of appropriate partners for co-cultivation remains a challenge. Furthermore, competitive interactions involving the suppression of BGCs or upregulation of known, functional metabolite(s) during co-cultivation efforts is also common. Herein, we performed an alternative approach for targeted selection of the best co-cultivation pair. Eight marine sediment-derived fungi were classified as strong or weak, based on their anti-phytopathogenic potency. The fungi were co-cultured systematically and analyzed for their chemical profiles and anti-phytopathogenic activity. Based on enhanced bioactivity and a significantly different metabolite profile including the appearance of a co-culture specific cluster, the co-culture of <i>Plenodomus</i>
<i>influorescens</i>
 (strong) and <i>Pyrenochaeta</i>
<i>nobilis</i>
 (weak) was prioritized for chemical investigation. Large-scale co-cultivation resulted in isolation of five polyketide type compounds: two 12-membered macrolides, dendrodolide E (<b>1</b>
) and its new analog dendrodolide N (<b>2</b>
), as well as two rare azaphilones spiciferinone (<b>3</b>
) and its new analog 8a-hydroxy-spiciferinone (<b>4</b>
). A well-known <i>bis</i>
-naphtho-γ-pyrone type mycotoxin, cephalochromin (<b>5</b>
), whose production was specifically enhanced in the co-culture, was also isolated. Chemical structures of compounds <b>1</b>
-<b>5</b>
 were elucidated by NMR, HRMS and [] analyses. Compound <b>5</b>
 showed the strongest anti-phytopathogenic activity against <i>Xanthomonas campestris</i>
 and <i>Phytophthora</i>
<i>infestans</i>
 with IC<sub>50</sub>
 values of 0.9 and 1.7 µg/mL, respectively.</div>
</front>
</TEI>
<pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">31979232</PMID>
<DateCompleted><Year>2020</Year>
<Month>10</Month>
<Day>13</Day>
</DateCompleted>
<DateRevised><Year>2020</Year>
<Month>10</Month>
<Day>13</Day>
</DateRevised>
<Article PubModel="Electronic"><Journal><ISSN IssnType="Electronic">1660-3397</ISSN>
<JournalIssue CitedMedium="Internet"><Volume>18</Volume>
<Issue>2</Issue>
<PubDate><Year>2020</Year>
<Month>Jan</Month>
<Day>23</Day>
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</JournalIssue>
<Title>Marine drugs</Title>
<ISOAbbreviation>Mar Drugs</ISOAbbreviation>
</Journal>
<ArticleTitle>Design of Fungal Co-Cultivation Based on Comparative Metabolomics and Bioactivity for Discovery of Marine Fungal Agrochemicals.</ArticleTitle>
<ELocationID EIdType="pii" ValidYN="Y">E73</ELocationID>
<ELocationID EIdType="doi" ValidYN="Y">10.3390/md18020073</ELocationID>
<Abstract><AbstractText>Microbial co-cultivation is employed for awakening silent biosynthetic gene clusters (BGCs) to enhance chemical diversity. However, the selection of appropriate partners for co-cultivation remains a challenge. Furthermore, competitive interactions involving the suppression of BGCs or upregulation of known, functional metabolite(s) during co-cultivation efforts is also common. Herein, we performed an alternative approach for targeted selection of the best co-cultivation pair. Eight marine sediment-derived fungi were classified as strong or weak, based on their anti-phytopathogenic potency. The fungi were co-cultured systematically and analyzed for their chemical profiles and anti-phytopathogenic activity. Based on enhanced bioactivity and a significantly different metabolite profile including the appearance of a co-culture specific cluster, the co-culture of <i>Plenodomus</i>
<i>influorescens</i>
 (strong) and <i>Pyrenochaeta</i>
<i>nobilis</i>
 (weak) was prioritized for chemical investigation. Large-scale co-cultivation resulted in isolation of five polyketide type compounds: two 12-membered macrolides, dendrodolide E (<b>1</b>
) and its new analog dendrodolide N (<b>2</b>
), as well as two rare azaphilones spiciferinone (<b>3</b>
) and its new analog 8a-hydroxy-spiciferinone (<b>4</b>
). A well-known <i>bis</i>
-naphtho-γ-pyrone type mycotoxin, cephalochromin (<b>5</b>
), whose production was specifically enhanced in the co-culture, was also isolated. Chemical structures of compounds <b>1</b>
-<b>5</b>
 were elucidated by NMR, HRMS and [] analyses. Compound <b>5</b>
 showed the strongest anti-phytopathogenic activity against <i>Xanthomonas campestris</i>
 and <i>Phytophthora</i>
<i>infestans</i>
 with IC<sub>50</sub>
 values of 0.9 and 1.7 µg/mL, respectively.</AbstractText>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Oppong-Danquah</LastName>
<ForeName>Ernest</ForeName>
<Initials>E</Initials>
<AffiliationInfo><Affiliation>GEOMAR Centre for Marine Biotechnology (GEOMAR-Biotech), Research Unit Marine Natural Products Chemistry, GEOMAR Helmholtz Centre for Ocean Research Kiel, Am Kiel-Kanal 44, 24106 Kiel, Germany.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Budnicka</LastName>
<ForeName>Paulina</ForeName>
<Initials>P</Initials>
<AffiliationInfo><Affiliation>GEOMAR Centre for Marine Biotechnology (GEOMAR-Biotech), Research Unit Marine Natural Products Chemistry, GEOMAR Helmholtz Centre for Ocean Research Kiel, Am Kiel-Kanal 44, 24106 Kiel, Germany.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Blümel</LastName>
<ForeName>Martina</ForeName>
<Initials>M</Initials>
<AffiliationInfo><Affiliation>GEOMAR Centre for Marine Biotechnology (GEOMAR-Biotech), Research Unit Marine Natural Products Chemistry, GEOMAR Helmholtz Centre for Ocean Research Kiel, Am Kiel-Kanal 44, 24106 Kiel, Germany.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Tasdemir</LastName>
<ForeName>Deniz</ForeName>
<Initials>D</Initials>
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<affiliations><list><country><li>Allemagne</li>
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Design of Fungal Co-Cultivation Based on Comparative Metabolomics and Bioactivity for Discovery of Marine Fungal Agrochemicals.

Design of Fungal Co-Cultivation Based on Comparative Metabolomics and Bioactivity for Discovery of Marine Fungal Agrochemicals.

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